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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in many tumors, which results in chromosome instability, and thus may add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical job in advertising and marketing cell-cycle development in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have already been described. Two supplemental splice variants ended up noted but in-depth nucleotide sequence data isn't still obtainable.
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Blocking peptides are peptides that bind particularly on the concentrate on antibody and block antibody binding. These peptide typically has the epitope regarded through the antibody.
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This click here tutorial outlines even more achievable optimization of cellular and immunoassay parameters to be certain the very best final results are...
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This guideline outlines even further achievable optimization of mobile and immunoassay parameters to guarantee the absolute best results are...
Antibodies certain to the blocking peptide no longer bind to the epitope within the target protein. By evaluating the staining from your blocked antibody as opposed to the antibody alone, one can see which staining is unique.
Focus on Info Cyclin E belongs for the really conserved cyclin loved ones, whose associates are characterised by a spectacular periodicity in protein abundance in the mobile cycle. Cyclins function as regulators of CDK kinases. Various cyclins show distinct expression and degradation styles which lead for the temporal coordination of each mitotic party. Cyclin E varieties a posh with and capabilities as being a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S changeover. This protein accumulates within the G1-S section boundary and is degraded as cells progress as a result of S phase.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally antibodies...
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